Well two washings and a lot of Shout stain remover took almost all of it out. Still a bit left where it was heavy quilted over. To answer questions on what I used: started out with disappearing ink pens but they seemed to run out of ink quickly, then tried the Dritz fat markings pencils but they had to be sharpened continuously, then tried the Dritz mechanical pencil that comes with the box of different colors and white chalk and that's what I used the most. I did use the green and blue colors. I honestly didn't think I would have a problem because when I marked my practice piece the color came off easily when I used a damp cloth and it was on Kona cotton. I will post a pic today of the finished quilt.