I tried eight colors of the roller-ball style Frixion and six colors of the highlighters.
All of them disappeared when I ironed them and all of them came back when I froze the fabric.
All of them washed out with a quick scrub in the sink and none of them came back after freezing.
I wouldn't hesitate to use any of them - I wash my quilts in the washer after I've finished them and that's a lot more thorough than the little hand-washing I gave my samples.