Embroidery thread or floss? Isacord is a 40wt machine embroidery thread and it is one of my all time favorites to use in the Longarm and I imagine it would work just fine hand quilting.
But if you are talking embroidery floss, the 6 strand stuff that comes in skeins commonly used for hand embroidery I can think of easier things to use to acheive the look you want. I think it would fray and break easily having to pull through all the layers unless you slid it down the needle a tad with every needleful of stitches. I have used 3 strands of floss and tied a quilt and it worked well but even with 3 strands and tying I had a lot of breakage from the strain put on it going through all those layers.