Try it out and see. Put your 4 inch Twister square on the 5 inch square on the back of the pieced top and trace around a few of the squares with a chalk marker. If the squares look too close for cutting, you haven't damaged the front of your fabric. I made myself a tiny twister ruler (way to small to rotary cut around) and ended up tracing the Twister shapes onto my pieced top and using my scissors to cut the marked blocks. It wasn't ideal but "where there's a will, there's a way."