No - they won't wash out - I am sleeping under a quilt that my Aunt Mary made at least 30 years ago - it was 18" cross stitch embroidery blocks that she did by hand. The blocks also had the quilting lines printed on them - my DD and I quilted this for her by hand following the printed lines. All of the X-stitch marks and the quilting lines are still printed in blue ink and the quilt has been washed umpteen times.