I wash hot and dry hot the batting, fabric and then the quilt. Learned my lesson when I gave a table runner to DD with out washing anything. DGS lost it at the table and the table runner did not survive the hot water wash to clean it up.
DD has a new washer with a sanitize setting and just had to wash a whole bed of linens and a bunch of clothes at that setting to clean up after a sick child and the I Spy duvet I made survived (and I think the water temp is hotter than normal hot).