Yes, you can do it ... but ... it depends on how heavily sprayed you did it.
And too, what sort of batting it is.
A gentle tug, and your backing may lift easily, or may not.
It may pull chunks of the batting with it though.
Especially if you heavily sprayed it.
If so, and you're not so much worried about salvaging your cheddar fabric,
... it may be easier to just bury it, covering it with your new backing!
Once removed, no problem ... just throw in the washer
... as the spray baste is meant to wash out of the fabric.