I'd take whichever one is nearer the kitchen. I like to know what's going on in my kitchen. :)

I'd choose the one closest to the kitchen and laundry area, that way you can multi-task, or get in a little sewing while doing other not so "fun" chores.

Lower level. I started with the suite upstairs, to much up and down. I now use smaller bedroom downstairs, very happy with it. Sun fades.

I would go with the one that is on the level where you would be the most. I like large and then I would invest in those natural lights (maybe they are just a advertising gimmic).

Big room. I do most of my sewing in the evening after dark anyway. So, light is not an issue. Stairs are though. If there is a frig and a bathroom on the level of the big room, there would be no reason to surface for long periods of time. Too much light can fade the fabric.

you can put lots into 75 square feet - go big, my dear, go big!

Can you "audition" the rooms before deciding? I know I feel most comfortable in the room I use for sewing, partly because of the natural light and I can also kind of keep an eye on whats going on in the rest of the house. When I got my large sewing cabinet it would have fit better in a different area of the house, but I wasn't comfortable sewing there. Just a thought.

Another vote for the larger room on the main floor! BTW, we want pics of the new sewing space!

ETA: Remember cutting tables, big boards and long arms take up a lot of space :D

Go big, you can always buy lights LOL

Hey, I would use both. The brighter, smaller room for my sewing/quilting and then whatever else I need in the other room.

For example, if you get a long arm it can go in the bigger room.

A guest room [the larger room] can always be functional for when there are no guests.

ali