Yes, you may use regular sewing thread. I did it for years. I would suggest that you use some sort of a stabilizar on the under side of the block, garment, etc. that you are putting the applique on. It will reduce the chances of the satin stitch "tunneling" or pulling together. It also works the same with blanket stitch, although it isn't as necessary. It depends on how wide you make the stitch in either case.
Happy a good time with the applique. It's lots of fun. :-D