I use the Gutterman's hand quilting thread....100% cotton.
I use a size 10 needle and have one of those Clover needlethreaders and LOVE it. It will actually thread those tiny needles.
The first quilt I hand quilted was a double bed size pieced top that my grandmother pieced and I found it in my mother's cedar chest.
Made from feed sacks and clothing. Have a good light. I use a 14 inch hoop. Have fun with it!